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INTRODUCTION TO BIOLOGY
By the end of this topic, you should be able to:
•Explain the meaning of the basic biological concepts and terminologies.
•Explain the importance of studying biology.
•Outline the characteristics of living things.
•Show the relationship between Biology and other related fields.
•Use sense organs to make correct observations.
•Take measurement of mass/ length and temperature
•Carry out simple Biology experiments
•Describe the Biology laboratory
•Distinguish the Biology from other school facilities
•Identify common apparatus and equipment used in Biology laboratory.
•Interpret warming sings on container of laboratory chemicals and apparatus Meaning of Biology
The word Biology is delivered from two Greek words, namely:
(i) Bios – means life
(ii) Logos – means study
Therefore,
Biology Is the study of life
OR
Biology: is a branch of science which deals with the study of living things.
A person who studies biology is called Biologist.
TERMINOLOGIES OF BIOLOGY
1. LIFE
Is the ability of living things to feed, reproduce, sense, respire, grow and develop, move and excrete.
2. CELL
Is a basic unit of life.
- Some living things have one cell hence called unicellular organisms while others have many cells hence called multicellular organisms
3. LIVING THING
Is anything which has life.
- Living things are also called organisms or animate things All living things are made up of very small units called cells.
Examples of living things
-Animals such as human being, cows, frogs, snakes
-Plants such as maize plants, bean plants
-Fungi such as mushrooms
-Microorganisms such as bacteria and viruses
- Protozoans such as amoeba, euglena, plasmodium and paramecium
BRANCHES OF BIOLOGY
There are two main branches of biology, namely
(i) Zoology
(ii) Botany
(i) ZOOLOGY
Is the study of animals.
Examples of animals
-Human beings
-Lizards
-Fish
-Cow
∆A person who study zoology is called Zoologist
(ii) BOTANY
Is the study of plants
Examples of plants
- Maize plants
-Bean plants
-Moss plants
- A person who study botany is called Botanist
OTHER BRACHES OF BIOLOGY
The following are other branches of biology:
1. MICROBIOLOGY
Is the study of microorganism
- Fern plants
- Pines, cypress and
- Christmas tree
Microorganisms are very small organisms that cannot be seen with naked eyes.
Examples of microorganisms
-Bacteria
-Viruses
∆ A person who studies microbiology is called microbiologist
2. BACTERIOLOGY
Is the study bacteria
Examples of bacteria
- Salmonella typhi
-Vibrio cholera
∆A person who studies bacteriology is called bacteriologist
3. VIROLOGY
Is the study of viruses Example of fungi
-HIV (Human Immunodeficiency Virus)
-Corona virus
-A person who studies virology is called virologist
4. MYCOLOGY
Is the study of fungi
Examples of fungi
- Yeast
-Mushroom
-A person who studies mycology is called mycologist
5. GENETICS
Is the study of inheritance
- A person who studies genetics is called geneticist
6. CYTOLOGY
Is the study of cells
- A person who studies cytology is called cytologist
7. ANATOMY
Is the study of internal structures of living things.
Example of internal structures of living things
- Heart
- Brain
- Kidneys -
Lungs
- A person who studies anatomy is called anatomist
8. ENTOMOLOGY
Is the study of insects
Example of insects
-Locust -
- Grasshoppers
- Butterflies
- Butterflies
- A person who studies entomology is called entomologist
9. PHYSIOLGY
Is the study of how body structures of an organism function
- A person who studies physiology is called physiologist
10. HOLTICULTURE
Is the science and art of growing fruits, vegetables, flowers or ornamental plants.
11. IMMUNOLOGY
Is the study of body immune system
- A person who studies immunology is called immunologist
12. PARASITOLOGY
Is the study of parasites
Example of parasites
-Plasmodium
- Some fungi
-Viruses
- Some bacteria
- A person who studies parasitology is called parasitologist
13. TAXONOMY
Is the study of classification
- A person who studies taxonomy is called taxonomist
14. ECOLOGY
Is the study of relationship between organisms and their environment
- A person who studies ecology is called ecologist
15. ORNITHOLOGY
Is the study of birds Examples of birds
- Hen
- Hawk
- Penguin
- Vulture
- A person who studies ornithology is called ornithologist.
16. ICHTHYOLOGY
Is the study of fish.
17. MORPHOLOGY
Is the study of external forms and structures of organisms
THE IMPORTANCE OF STUDYING BIOLOGY
The following is an outline of why the study of biology is important:
(i) It helps us to understand ourselves better.
(ii) Biology helps us to understand our environment better and how to conserve it
(iii) Biology helps us to know the causes, transmissions, signs and symptoms of diseases.
(iv) Biology enables scientists to come up with ways of preventing, treating and curing diseases.
(v) Biology enables researchers to come up with improved breeds of crops and animals.
(vi) It is a foundation for getting into careers such as medicine and agriculture.
(vii) Biology helps us to answer some fundamental questions such as, what do we need to survive?
(viii) Biology helps us to get professional people like doctors, pharmacists and teachers.
(ix) It encourages international cooperation.
(x) It helps us to acquire research skills such as measuring, observing, analysis and making conclusions.
QUESTION
1. Form one students in a certain secondary school were interested to know why it is necessary to study biology. Assume you are a Biology teacher: educate these students on the importance of studying Biology by giving six (6) points.
2. Outline six reasons why it is important to study Biology?
3. Biology is more than a study of animals and plants. Explain
4. Distinguish between unicellular organisms from multicellular organisms giving an example in each case.
5. How Botany differs from Zoology?
CHARACTERISTICS OF LIVING THINGS
There are seven (7) characteristics (life processes) that distinguish living things from nonliving things.
These are: -
(i) Movement and locomotion
(ii) Respiration
(iii) Nutrition
(iv) Irritability or sensitivity
(v) Growth
(vi) Excretion
(vii) Reproduction
NB: For easy study of these characteristics the word MR NIGER can be used
(i) MOVEMENT
Is the act of changing position or postures by the whole organism or part of an organism.
- Living things moves in order to search for food, water, mate, light and to escape from dangers.
- It can occur at a cellular level e.g. cytoplasmic streaming in amoeba and it can also occur in an organ level. E.g. Closing and opening of leaves, folding of leaves in plants.
Example of movement
- Singing
- Closing and opening
- Yawning of leaves
- Clapping hands
LOCOMOTION
Is the change in position by the whole body of an organism from one place and another. Example of locomotion
- Walking - Swimming
- Running - Crawling
(ii) RESPIRATION
Is the process of breaking down of food materials within the cells to release energy.
- Living things need energy for movement, growth and development, and functioning of body organs.
(iii) IRRITABILITY (SENSITIVITY)
Is the ability of an organism to detect and respond to a stimulus.
Stimulus
Is a change in the external and internal environment of an organism.
Examples of stimulus
- Light
- Temperature
- Cold
- Wind
-An alarm clocks
- A smell of food
- A fly landing on your eye.
Response
Is the change shown by an organism in reaction to a stimulus
Example of response
- Salivation when food smells
- Blinking when a fly landing on the eye
- Picking up a phone after hearing it ringing
(iv) NUTRITION (FEEDING)
Is the process of taking in food, absorb and assimilate nutrients
- Nutrition is the characteristic of living things which is the basic difference between plants and animals.
- Plants make their own food through photosynthesis process hence called autotrophs
- Animals get their food by eating other living things hence called heterotrophs.
- All living things need food to provide energy for such activities such as growth, repair and health.
(v) GROWTH AND DEVELOPMENT
Growth: Is an irreversible and permanent increase in size and dry mass of an organism.
- All living things grow and build up their bodies.
- Animals grow until they reach certain adult size, but most plants can grow continuously throughout their lives.
Example of growth
- Increase in body weight
- Increase in body height
- Increase in body size and shape
DEVELOPMENT
Is the change in the complexity of an organism.
Example of development
- Emergence of a new structure
- Ripening of fruits.
(vi) REPRODUCTION
Is the process by which living things produce new individuals of their kind.
- All living things reproduce, to replace organisms lost by death.
- Human beings bear babies; birds hatch chicks; and plants produce seedlings as new organisms, which eventually grow to mature organisms to replace those lost by deaths.
(vii) EXCRETION
Is the process of removing metabolic waste products from the body of living organisms.
- Excretion is shown by both animals and plants
Example of waste products
- Carbon dioxide
-Urea
- Excess water
-Ammonia
- Waste products are removed from the body by excretory organs such skin, kidneys, lungs and liver
DIFFERENCES BETWEEN LIVING THINGS AND NON-LIVING THINGS
Living things | Non-living things | |
They respire | Do not respire | |
They grow | Do not grow | |
They respond to stimuli | Do not respond to stimuli | |
They reproduce | Do not reproduce | |
They excrete | Do not excrete | |
They feed | Do not feed | |
They move | Do not move | |
QUESTIONS
1. A scientific space craft brought a material to earth from outer space. Explain how you would establish if the material is living or non –living thing.
Give four points. Your answer should be in tabular form as shown below:
2. By giving example distinguish the following terms:
(a) Nutrition and respiration
(b) Growth and development
(c) Movement and locomotion
3. Excretion is the process of removing metabolic by-products from the body of an organism. Give four examples of excretory products eliminated by the living things.
4. Write seven processes that take place in the bodies of living things.
5. State whether it is movement or locomotion.
(a) Singing (e) Hopping
(b) Yawning (f) Leaping
(c) Clapping hands (g) Flying
(d) Walking
RELATIONSHIP BETWEEN BIOLOGY AND OTHER RELATED FIELDS
Biology relates with many other fields, some of them are:
(i) Agriculture
(ii) Medicine
(iii) Pharmacy
(iv) Nutrition
(v) Forestry
(vi) Veterinary science
THE TABLE BELOW SHOW HOW BIOLOGY RELATES WITH OTHER FIELDS
FIELD | MEANING | HOW IT RELATES TO BIOLOGY |
AGRICULTURE | Is the practice of growing crops and keeping animals An expert person in agriculture who gives advice to farmers is called agriculturist | Knowledge of Biology enables researchers to come up with improved breeds of crops and animals in order to increase animal products such as meat, milk and eggs.. |
MEDICINE | Is the science of diagnosing, treating and preventing diseases in humans | (i) Doctors, surgeons and nurses apply knowledge of biology to diagnose, treat and prescribe the right medicine Page 8 of 135 to cure the disease. (ii) Doctors apply knowledge of biology to offer education to the patients on how to prevent themselves from the diseases |
NUTRITION | Is the study of how food is taken in and used in the human body in relation to the needs of the body. A person who studies nutrition is called nutritionist. | Knowledge of biology enables us to know the suitable diet that should be taken in order to maintain good health |
PHARMACY | ||
VETERINARY SCIENCE | Is the study concerned with the health of animals and treatment of diseases that affect them. A person who treats and taking care of the health of animals is called veterinarian | Veterinarians apply knowledge of biology to diagnose, treat and prevent illness in large and small animals |
FORESTRY | Is the science or practice of planting and taking care of trees and forests. - A forest is a large area of land covered with trees. A person who takes care of forests is called a forester. | Knowledge of biology helps to know how to plant trees, the importance of planting trees and the danger of cutting down of trees Knowledge of biology is applied to determine the type of plants the type of plants to be grown related to the climate, soil and water availability. |
SCIENTIFIC PROCESSES IN BIOLOGY
The study of biology involves scientific processes.
- Scientific processes are conducted in order to solve different life problems and help us to understand well our environment.
WAYS USED IN CARRYING OUT SCIENTIFIC PROCESSES IN BIOLOGY
There are three basic skills that are needed in studying biology (scientific studies)
(a) Observation
(b) Measurement
(c) Scientific method
(a) OBSERVATION
This method is done by using sense organs
- All scientific investigations involve observation using sense organs
SENSE ORGANS USED IN OBSERVATION
There are five sense organs used in observation, namely;
(i) Eyes
(ii) Ears
(iii) Nose
(iv) Tongue
(v) Skin
THE FUNCTION OF EACH SENSE ORGAN
EYES
Used for seeing or vision.
Example: shape, colour and size of the object
SKIN
Used for touch
Used to detect pain, heat, cold and pressure
Example: texture of an object, temperature and sharpness of an object.
NOSE
Used for smelling.
Example: smell of chemicals or foods
EARS
Used for hearing.
Example: sounds of living and non-living things
TONGUE
Used for tasting.
Example: tastes of different specimens such as sugar and salt.
Example: If you have a coconut, you can use sense organs to determine that:
(i) It is round – by seeing
(ii) It contains a liquid – by hearing the sound when shaken
(iii) It has rough husk (surface) – by touching
(iv) It has good smell – by smelling
(v) It has good taste – by tasting after breaking the coconut
WEAKNESS OF USING SENSE ORGANS IN MAKING OBSERVATION
(i) Sense organs are based on person's ideas rather than facts.
(ii) Sense organs alone cannot be enough to give out all the details in accuracy way.
ADVANTAGE OF USING SENSE ORGANS IN OBSERVATION
- Enables us to study living things.
PRECAUTIONS WHEN USING SENSE ORGANS IN OBSERVATION
(i) Avoid smelling everything in the laboratory without permission from your teacher or laboratory technician.
(ii) Avoid tasting anything in the laboratory unless you are told do so by the teacher or laboratory technician.
(b) MEASUREMENT
Is the system of using specific instruments and units to make investigations. Or is the process of assigning numerical values to different things in order to find their size or magnitude.
- For any meaningful measurement in Biology, we need to state the physical quantity (parameter) to be measured and its units.
- The units applied in measurement are International System of units (SI-Units).
COMMON PHYSICAL QUANTITIES (PARAMETERS) MEASURED IN BIOLOGY
The following are common physical quantities (parameters) measured in biology with their standard units and instrument used for measuring each parameter:
PHYSICAL QUANTINTY | Instrument used | SI unit |
(i) Mass | Weighing balance | kilogram (kg) or gram (g) |
(ii) Length | Ruler or tape measure | Metre (m) or centimetre (cm) |
(iii)Temperature | Thermometer | Celsius (0C) or Kelvin (K) |
(iv) Time | Stopwatch | Seconds (s) |
(v) Pulse rate | Stethoscope | Numbers of beats per minutes |
1. LENGTH (l)
Is the distance between any two points
- The instrument used to measure length is called ruler or tape measure
- It is expressed in millimetres (mm), centimetres (cm), metres (m), and kilometers (km)
Where:
1cm = 10 mm
1m = 100 cm
1km = 1000 m
WORKED EXAMPLES
1. Convert the following units into millimeter
(i) 10 cm
(ii) 10 km
(iii) 50 m
2. Change the following units as directed
(i) 1000km to cm
(ii) 320 to km
(iii) 200mm to km
(iv) 900km to m
2. MASS (m)
Is the quantity (amount) of matter in the object
The instrument that can be used to measure mass is called weighing balance such as beam balance, electronic balance
It is expressed in grams (g) or kilograms (kg)
Where
1kg = 1000g
WORKED EXAMPLES
1. Convert the following units as directed
(i) 70g into kg
(ii) 40g into kg
(iii) 10kg into g
3. TEMPERATURE
Is the measure of relative hotness and coldness of a body or object
- The instrument used to measure temperature is called thermometer
- It is expressed in Kelvin (K), degrees Celsius (℃) and degrees Fahrenheit (℉)
- The normal body temperature is between 36℃ to 37℃
Temperature in different units can be calculated by using the following formula
(i) K = ℃ + 273
(ii) ℉ = ( × ℃) + 32
(iii) ℃ = × (℉ − 32)
WORKED EXAMPLES
1. Convert the following units into Kelvin (K)
(i) 85℃
Solution
From: K = ℃ + 273
Where: ℃ = 85
K = 85 + 273 = 358
Therefore: 85℃ = 𝟑𝟓𝟖K
(ii) −450℃
Solution
From: K = ℃ + 273
Where: ℃ = −450
K = −450 + 273 = −177
Therefore: −𝟒𝟓𝟎℃ = −𝟏𝟕𝟕K
(iii)115℃
(iv) 40℉
2. Convert the following units into degrees Celcius (℃)
(i) 20K
(ii) 40℉
(iii)100℉
3. Convert the following temperature into degrees Fahrenheit (℉)
(i) 85℃ (iii)100℃
(ii) 115℃ (iv) 20K
4. PULSE RATE
Is the number of times the heart beats per minute.
- The instrument used to measure pulse rate is called stethoscope
- It is expressed in number of heart beats per minute.
NB: For a person in normal health, the pulse rate occurs 72 times per minute but for the embryo of human being aged about 60 days, the pulse rates is 150 beats per minute
Parts of the body where Pulse is felt
Pulse is felt in any artery by placing the index and middle fingers on:
- The neck
- Behind the knee,
- The wrist
- On the side of elbow
- Below ears
- Near the ankle joint.
NB: Do not use the thumb to make pulse because it has its own pulse.
(c) SCIENTIFIC METHOD
Is a set of steps that a scientist follows to solve a problem about nature.
OR is an organized set of guidelines used to solve scientific problem
- The scientific method is also called scientific process or scientific procedure
IMPORTANCE OF SCIENTIFIC METHOD
(i) It helps us to solve scientific problem
(ii) It helps us to gain new knowledge
(iii) It helps us to conduct project work or carry out field work
STEPS OF THE SCIENTIFIC METHOD
The scientific method involves the following steps;
1. Identifying a problem
2. Formulating a hypothesis
3. Conducting experiment
4. Collecting data
5. Analysing data
6. Interpreting data
7. Conclusion
1. IDENTIFYING THE PROBLEM
This is the first step in the scientific method where a problem is recognized or identified.
- In our day to day life one often comes across questions which require explanations or answers.
PROBLEM
Is a specific event observed in the environment.
For example
In a certain region of Tanzania, Agricultural officers observed tomatoes yield was low despite frequent irrigation, correct planting techniques, timely planting and adequate sunlight.
Question (problem could be)
What could have caused poor yield of tomatoes in Jama's garden despite all the efforts made?"
2. FORMULATING A HYPOTHESIS
This is the second step in the scientific method where a possible answer to a question is suggested.
Hypothesis
Is an intelligent guess which tries to answer the observed problem.
Or is a tentative explanation for the observation made.
- A hypothesis from the above problem could be that the low yield of tomatoes is caused by poor soil fertility."
- Therefore, the agricultural officers suggested that application of fertilizer could increase the tomatoes yield.
Ways used to test hypothesis
(i) By careful observation in a natural setting
Example: Behaviour of a certain type of wild animal can be studied by carefully observing the animal in the field they live
(ii) By conducting experiment
3. EXPERIMENTATION
In this step, scientists plan and conduct an experiment
- In planning an experiment
- Scientists collect necessary information concerning the problem, determine which apparatus will be used, which materials will be required and what procedures will be followed to prove or reject the hypothesis.
Experiment
Is a test done to confirm or reject the hypothesis
- Experiment is conducted to determine if the hypothesis is correct or not.
- Experiment is repeated more times to obtain more accurate results
NB: In conducting experiment, two sets of subjects are involved:
(a) Experimental group or test experiment
(b) Control group or control experiment
(a) Test experiment
Is the one that receives treatment
Or is the experiment which is done by changing variables or factors from time to time
Example: addition of fertilizers (b) Control experiment
Is the one that does not receive treatment
Or is the experiment which is done without changing variables or factors.
Example: no addition of fertilizers
- Control experiment is usually set in order to compare it with the test experiment and to prove the hypothesis.
From our example of low tomatoes yield, a plan for investigation could be as follows: Aim of the experiment: To observe if low yield is due to poor soil fertility Requirements: Tomato seed from affected area, fertilizer Instructions:
(i) Set aside two plots and name them as: Plot A and Plot B
(ii) Subject all the two plots to the same ecological conditions
(iii) In plot A apply fertilizer as recommended by the agricultural officers
(iv) In plot B fertilizer should not be added.
(v) Harvest and compare the yields of the two plots
NB: In the above planned experiment, the plot B is not applied with fertilizer, this is a control experiment. The controlled experiment proves that the fertilizer is the one that determines the tomatoes yield.
The experiment is conducted by using variables
VARIABLES
Are conditions or factors that can change or be changed during an experiment
TYPES OF VARIABLES
There are three types of variables, namely;
(a) Dependent variable
Is the condition or factor that is investigated or observed to obtain the results of the experiment
(b) Independent variable (manipulated)
Is the condition or factor that is changed systematically to obtain different results
(c) Controlled variable
Is the condition that is kept constant during the experiment. Example light
4. COLLECTING DATA
Is the process of recording and presenting all the changes or information observed during experiment
- In this step, all changes observed during the experiment are collected and recorded.
Data
Is the information gathered during the experiment.
Or is unprocessed information that is collected from the experiment by observation or measurement.
5. DATA ANALYSIS
Is the process of translating information observed during an experiment.
- Data analysis involves comparing the results obtained and hypothesis
- Data are organized and analyzed in order to test the hypothesis, to see trends and patterns
- To enable one to see the results in an organized manner, data should be presented using different methods such as tables, bar charts, pie charts, histograms drawings or line graphs.
Example
A table below presents the data analyzed from our tomato experiment
PLOT TOMATO YIELD
A 2,300 kg
B 150 kg
6. DATA INTERPRETATION
Is the process of giving meaning of the collected data.
- In our tomato yield experiment, the data in the table above shows that there is a difference in tomato yield from the two plots.
- The yield of plot A was higher than of the plot B. this mean that, the amount of fertilizer has effect on the amount of tomato yield.
7. CONCLUSION
Is the statement that summarizes the results of the experiment conducted.
- In conclusion, a hypothesis can be accepted or rejected based on the data collected and analyzed. Example
- In our tomato yield experiment, the results have shown that the application of the right amount of fertilizer increased crop yield.
- Therefore, low tomato yield was a result of poor soil fertility.
- The results support the hypothesis that low crop yield was caused by poor soil fertility
- In this case, the hypothesis was accepted or confirmed.
- In some cases, when the hypothesis selected and involved for experiment is rejected another hypothesis is formed and an experiment is conducted again.
- Conclusion results to the formation of law, theory and principle. The steps for scientific method are summarized in the figure below.
The steps for scientific method are summarized in the figure below.
CONCLUSION
THE BIOLOGY LABORATORY
The word 'laboratory' originates from Latin name 'laborare' which means "a place for work"
Laboratory is a room or building designed for carrying out scientific experiments.
There are various types of laboratory such as;
(i) Biology laboratory
(ii) Chemistry laboratory
(iii) Physics laboratory
(iv) Clinical laboratory
(v) Industrial laboratory
BIOLOGY LABORATORY
Biology laboratory is a special room or building where biological experiments are conducted.
The biology laboratory differs from other scientific laboratories in the following ways;
(i) Biology laboratory has preserved specimens
(ii) Biology laboratory has models of organs
(iii) Biology laboratory has microscope
(iv) Biology laboratory has dissecting kit
(v) Biology laboratory has charts showing different body systems and organs
(vi) Biology laboratory has cages Cage is an enclosure made to hold animals
(vii) Biology laboratory has aquaria (single–aquarium) Aquarium is tank made for keeping live aquatic animals
QUALITIES OF A GOOD LABORATORY
(i) Laboratory has doors which exit outwards. This is for easy escape when accident occurs.
(ii) Laboratory should have source of water. Water help in boiling, mounting specimens and cleaning.
(iii) Laboratory should have source of heat. Heat is needed for experiments
(iv) Laboratory should have electricity supply. Electricity is for lighting and electronic devices.
(v) Laboratory should have adequate apparatus for conducting experiments
(vi) Laboratory should have adequate storage room and cupboards. For storing apparatus and specimens
(vii) Laboratory should have adequate space for carrying out experiments.
(viii) Laboratory should have emergency facilities placed in area where can be reached easily. Example fire extinguishers and sand buckets
LABORATORY SAFETY RULES
Laboratory can be a dangerous place if the safety rules are not observed.
The following are some rules to ensure safety in a laboratory.
(i) Do not enter the laboratory or do an experiment without permission from a teacher.
(ii) Do not run, play or make noise in the laboratory. (iii) Do not eat any food or drink in the laboratory
(iv) Do not use broken apparatus or unlabeled chemicals.
(v) Do not take any laboratory tool or specimen out of the laboratory.
(vi) Do not taste anything without being instructed
(vii) Do not put laboratory equipment at the edge of benches or tables.
(viii) Do not leave water and gas taps open
(ix) Do not block the emergency facilities and passage ways.
(x) Follow all instructions before starting any experiment.
(xi) Wash your hands with clean water and soap after the experiment.
(xii) Wash the apparatus and benches at the end of the experiment
(xiii) Wear safety tools before you start the experiment
(xiv) Read and understand all the warning signs on the container.
(xv) Report all accidents occurred to your teacher or laboratory technician.
COMMON APPARATUS AND EQUIPMENT USED IN THE BIOLOGY
LABORATORY
APPARATUS
Is a tool used for carrying out experiments in laboratory.
Some common biology laboratory apparatus and equipment include:
1. TEST TUBE
It is used to hold, mix or heating small amounts of substance
2. TEST TUBE HOLDER
It is used to hold test-tubes during heating
3. TEST TUBE RACK
Used to hold and support test tubes
4. PETRI DISH
- Used for putting specimens
- Used for growing small organisms
5. BUNSEN BURNER
Used to heat materials in the laboratory
6. THERMOMETER
Used to measure temperature
7. DROPPER
Used for adding liquids drop by drop
8. BEAM BALANCE
Used to measure mass of substances
9. MORTOR AND PESTLE
Used for crushing and grinding substances
10. MICROSCOPE
Used to magnify micro-organisms
11. HAND LENS
Used to magnify small organism/objects for observation.
12. POOTER
Used for catching up small organisms without hurting them
It is made with two tubes where one is for sucking organisms and another for drawing in the apparatus
13. FISH NET
Used for catching organisms living in water Example fishes and tadpoles
14. SWEEP NET
Used for catching flying insects
15. SPECIMEN BOTTLE
Used for keeping preserved organism
16. DISSECTING KIT
It carries tools used for dissection
THE MICROSCOPE
MICROSCOPE: Is an instrument used to magnify micro-organism.
Micro-organisms are very smallest organism which cannot be seen by our naked eyes.eg bacteria.
TYPES OF MICROSCOPE
There are two types of microscope
(i) Light microscope (compound)
(ii) Electron microscope
1. ELECTRON MICROSCOPE
Is a microscope that uses beams of electrons to magnify specimen.
The maximum useful magnification of most electrons microscope is one million times (100, 0000×)
Electron microscope is very expensive.
It is mostly found in research centres.
2. LIGHT MICROSCOPE
Is a microscope that uses light to illuminate and magnify small specimen. Light microscope is also called compound microscope
It has low magnification compared to that of electron microscope
Maximum useful magnification of most light microscope is about one thousand time (1000×).
Light microscope is commonly found in school laboratories and health centres.
DIFFERENCES BETWEEN ELECTRON AND LIGHT MICROSCOPE
LIGHT MICROSCOPE | ELECTRON MICROSCOPE |
(i) It is less expensive | It is more expensive |
(ii) Uses light to illuminate object | Uses electron beam to illuminate object |
(iii)It has low magnification | It has high magnification |
(iv) It has low resolution | It has high resolution |
(v) It uses glass lenses | It uses magnetic deflector |
A WELL LABELLED DIAGRAM OF A LIGHT MICROSCOPE
PARTS OF THE LIGHT MICROSCOPE AND THEIR FUNCTION
Light microscope has the following parts:
(i) Eyepiece lens (ocular lens)
(ii) Eyepiece (ocular)
(iii) Body tube
(iv) Coarse adjustment knob
(v) Fine adjustment knob
(vi) Rotating nose piece
(vii) Objective lens
(viii) Stage
(ix) Stage clip
(x) Condenser
(xi) Diaphragm
(xii) Mirror
(xiii) Arm
(xiv) Hinge screw
(xv) Base
PART OF THE MICROSCOPE | FUNCTION | |
1. Eye piece lens | Used to increase magnification | |
2. Ocular tube | Allow light to pass from the objective lens to the eyepiece. It has a lens that magnifies the specimen | |
3. Body tube | It holds the ocular lens and objective lenses at the proper working distance from each other. It supports the eyepiece and rotating nose piece It connects the eyepiece to the objective lens | |
4. Coarse adjustment knob | It raises or lowers the body tube in order to bring the image into focus. | |
5. Fine adjustment knob | It raises or lowers the body tube in order to bring the image into sharp focus | |
6. Rotating nose piece | It holds two or more objective lenses | |
7. Objective lens | It brings s image into focus and magnify it | |
8. Stage | It is a place where slides of specimen are kept | |
9. Stage clip | It holds the slides firmly in place. | |
10. Condenser | It concentrates the light which passes through the specimen. | |
11. Diaphragm | It regulates the amount of light that passes through the specimen. | |
12. Mirror | It reflects and directs light to the specimen under observation. | |
13. Hinge screw | It raises or lowers the stage to keep the specimen in right position for observation. | |
14. Arm/limb | Supports the body tube, knobs, stages, diaphragm and mirror | |
It helps to handle microscope when moving it. | ||
15. Base | It provides firm support for the microscope. |
HOW TO USE LIGHT MICROSCOPE
(i) Place the microscope on the laboratory bench or table. Position the microscope such that the arm faces you and the stage faces away from you.
(ii) Mount the specimen on slide and cover it with a cover slip.
(iii) Make sure that the low objective lens is in line with the eye piece lens.
(iv) Place the slide with the specimen on the stage and hold it in place with the stage clip.
(v) While looking through the eyepiece use your hand to adjust the mirror so that the light is directed to the specimen on the stage. Always keep both eyes open when looking through eyepiece
(vi) Adjust the coarse adjustment knob to bring the specimen into focus (vii) Adjust the fine adjustment knob to bring the specimen into a sharp focus.
(viii) Rotate the nose piece to a higher power objective lens if you want to observe more details of the specimen.
PRECAUTIONS WHEN HANDLING AND CARING OF A LIGHT MICROSCOPE
(i) Always use two hands when carrying a light microscope. One hand holding the arm and the other holding the base.
(ii) Never place the microscope at the edge of the bench or table. This is because it could be knocked over.
(iii) Do not touch the surface of the mirror or lenses with your fingers.
(iv) Clean dirty lenses using lens tissue or a soft cloth
(v) Lubricates the moving parts regularly
(vi) Always use the cover slip in order to protect the lenses of the microscope from getting into contact with the specimen
(vii) Remove the slides from the stage immediately after use.
(viii) Turn the adjustment knob slowly. Always start with the course adjustment knob.
(ix) When not in use, cover the microscope with clean cloth and store it in dry and dustless place.
(x) When not in use for a long time, remove the lenses and put them in a desiccator (xi) Move the objective lenses up when storing the microscope.
(xii) When storing the microscope rotate the nose piece so that the low magnification lens is in line with the eyepiece lens.
NB: SLIDE
Is a flat rectangular piece of glass used to hold a specimen for microscopic observation
SLIDE COVER (COVER SLIP)
Is a small glass used to cover the specimen on slide
MAGNIFICATION
Is the number of times the image has been enlarged
- In microscope, magnification is determined (calculated) by multiplying the eye piece lens magnification by the objective lens magnification.
Total magnification = Eyepiece lens magnification × Objective lens magnification |
Worked Example
1. Calculate the total magnification of the image seen by ×5 of eyepiece lens and by ×10 of the objective lenses
Solution
Data given
Total magnification =Asked
Eyepiece lens magnification = 5
Objective lens magnification =10
Total magnification = eyepiece lens magnification × objective lens magnification
Total magnification = 5 ×10 =50
Therefore, the total magnification of the image is ×50
2. Calculate the objective lens magnification of the object, if the total magnification is ×250 and the eyepiece lens is ×10
Solution
Data given
Total magnification =250
Eyepiece lens magnification = 10
Objective lens magnification =Asked
From:
Total magnification = eyepiece lens magnification × objective lens magnification
250 = 10 × objective lens magnification
Objective lens magnification = = 25
Therefore, the objective lens magnification is ×25
REVISION QUESTIONS
1. Calculate the total magnification of image, if the objective lens magnification is ×20 and eyepiece lens magnification lens is ×10.
2. If the magnification power of eyepiece lens of a compound microscope is ×10 and that of the high-power objective lens is ×50, what is the total magnification of the specimen under observation?
3. Calculate the objective lens magnification of object. If the total magnification of the object is ×300 and the eyepiece lens magnification is ×5.
4. Calculate the eyepiece lens magnification of an object. If the objective lens magnification of the object is ×15 and the total magnification of an object is ×120.
HAND LENS
Is an instrument composed of a convex lens used for magnifying small objects.
The Diagram of hand lens
PARTS OF HAND LENS
Hand lens has three main parts, namely:
(i) Convex lens: used for magnifying small objects.
(ii) Frame: gives support to the convex lens
(iii)Handle: helps to handle the hand lens when moving it.
HOW TO USE A HANDLENS
In order to magnify an object by using a hand lens, the following procedures should be followed:
(i) Place the object or specimen under observation on the bench or table.
(ii) Move the hand lens with one hand from the object or specimen to the eye
(iii)As the object or specimen comes into focus an enlarged image will be observed
How to determine magnification of an object or specimen viewed under a hand lens Magnification of an object or specimen viewed under a hand lens is determined (calculated) by dividing the length of a drawing by the length of the actual object.
As follows:
Drawing magnification =
OR
Magnification of a drawing =
WORKED EXAMPLES
1. A drawing of 10cm in length was made of Bee of 5cm in length. Calculate the magnification of drawing.
Solution
Data given
Length of a drawing =10
Length of the actual object = 5
Magnification of a drawing =Asked
From:
Length of a drawing
Magnification of a drawing =
Length of the actual object
10cm Magnification of a drawing = = 2
5cm
Therefore, Magnification of a drawing is ×2
REVISION QUESTIONS
1. If the length of the drawing is 8cm and the actual length of the object is 2cm. What will be the magnification of a drawing?
2. (a)How do you determine the magnification of an object viewed under a hand lens?
3. Basically, there are two types of microscope, name them:
4. Compare and contrast a light microscope and an electron microscope.
5. How do you determine the magnification of an object viewed under a light microscope
6. Mention three parts of a hand lens
7. (a) Draw a well labelled diagram of a light microscope.
(b) State the function of each part labelled in the diagram 7 (a) above.
8. Draw a well labelled diagram of a hand lens.
The following are warning signs that are found on apparatus and chemical containers in the laboratory or on the boxes in which chemicals and apparatus are kept.
(i) Toxic
(ii) Corrosive
(iii) Flammable
(iv) Oxidant
(v) Explosive
(vi) Harmful
(vii) Irritant
(viii) Fragile
(ix) Radioactive
(x) Biohazard
1. TOXIC
Is a poison substance which may cause death when touched, swallowed or inhaled.
Examples of toxic substances
- Pesticides
- medicine
A diagram of toxic warning sign
2. CORROSIVE
Is a substance that can burn the skin or a surface.
= It can cause body wounds or burn various things such as a clothes and furniture
Examples of corrosive substances
- Concentrated acids e.g. sulphuric acid,
- Concentrated alkaline e.g. Sodium hydroxide.
A diagram of corrosive warning sign
3. FLAMMABLE
Is a substance which can catch fire easily.
- The substances should never be brought near open flame
Examples of flammable materials
- Alcohol
- Petrol
- Kerosene
- Diesel
A diagram of flammable warning sign
4. OXIDANT
These are chemical that can accelerate burning of materials.
Example of oxidant substances
- Potassium permanganate
- Potassium hydroxide
A diagram of oxidant warning sign
5. EXPLOSIVE
An explosion is a forceful rapid reaction, which involves throwing off of particles at high speed.
Example of explosive substances
- Gunpowder
- Mosquito spray
6. HARMFUL
Is the substance which can cause illness.
This substance may not kill immediately but may have effects when used for a long period.
Examples of harmful substances
-Alcohol
- Cigarettes
DIAGRAM OF HARMFUL
7. RADIOACTIVE
Is the substance that emits harmful radiations.
- Radioactive substances are elements that emit radiations either alpha, beta, gamma or neutrons.
- Radioactive substances can cause dangerous effects such as skin damage, skin diseases, disabilities, death, blindness and various types of cancer ases Example of radioactive
- X- rays
- Uranium splits to emit neurons
- Radium emits alpha and gamma rays
- Tritium emit beta rays
- Cesium emit gamma rays
A diagram of radioactive warning sign
BIOHAZARD
Is a biological substance that can cause disease or threat to the health of humans.
- Biohazard contains microbes
- Biohazard is a short form of biological hazard
Example of biohazards substances
- Medical waste e.g. wound dressings
- Sludge waste
DIAGRAM OF BIOHAZARDS
REVISION QUESTIOS
1. Write TRUE or FALSE
(i) A laboratory is a special room designed for scientific investigation
(ii) Thermometers and burners are only found in the biology laboratory
(iii) Laboratory safety rules are formulated to frighten students
(iv) Corrosive chemicals can burn books and clothes because they catch fire easily
(v) Using microscope, biologists have been able to observe very small organisms.
2. Name the laboratory apparatus used in the following activities
(i) Magnifying substance
(ii) Catching flying insects
(iii) Keeping specimens
(iv) Crushing things
(v) Measuring mass
3. State the function of the following parts of microscope
(i) Base
(ii) Diaphragm
(iii) Condenser
(iv) Mirror
(v) Stage
(vi) Objective lens
4. List four features that make biology laboratory different from other facilities
5. Explain how the following substances should be handled
(i) Harmful
(ii) Explosive
(iii) Oxidants